Liza Meutia Sari, Gus Permana Subita and Elza Ibrahim Auerkari
Syiah Kuala University, Indonesia
Posters & Accepted Abstracts: Primary Health Care
Many herbs have been discovered to be potential sources of anticancer drugs. Biji Pinang or Areca nut has a high content of phenolics and flavonoids, which is related to antioxidant activity. However, data on its effects on oral squamous cell carcinoma, human keratinocyte cells and the safety of its use is not available. The objective of this study is to identifiy the phytochemical composition of Areca nut, antioxidant activity, and citotoxicity activity in HSC-2, HSC-3, and HaCat. The Areca nut was extracted by ethanol 96%, phytochemical screening, LC-MS, DPPH assay, and MTS assay. The presence of alkaloid, tannin, flavonoid, catechin, and quercetin compounds in Areca nut extract. Total phenolic content was 80.3 mgTAE/g and total flavonoid content was 238.5 mgCE/g. The EC50 was 15.95�±0.84 �¼g/ml. The extract of Areca nut showed higher toxicity on HSC-3 cell compared to HSC-3 IC50 was 164.06 �¼g/ml vs. 629.50 �¼g/ml in HSC-2. The proliferation effect was found in HaCat cells. The antioxidant activity of the extract of Areca nut might be associated with the presence of catechin, selective cytotoxicity on HSC-2 and HSC-3, and HaCat cells.
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