Department of Medical Biology, Ankara Yildirim Beyazit University, Ankara, Turkey
Research Article
Circulating Tumor Cells as a Liquid Biopsy Biomarker in Patients with Breast Cancer
Author(s): Bilgesu Ersan Yazici, Ozen Ozensoy Guler, Ahmet Carhan, Elif Ercan and Ender Simsek*
Background/aims: Despite state-of-the-art diagnostic/therapeutic approaches, cancer accounts for the majority of mortality rate annually, and patients with breast cancer are not exception. So, a comprehensive understanding on the biological activities of Circulating Tumor Cells (CTCs) seems as a more effective cancer biomarker. This study aims to investigate the importance of detected CTCs for identification of metastatic cells in patients with breast cancer.
Materials and methods: Peripheral blood samples were collected from 11 patients with breast cancer, and 8 healthy volunteers. CD45 coupled magnetic microbeads were used to enrich CTCs. Then, the cells were exposure with fluorochrome-labeled antibodies, being cultured in an optimized condition, and subsequently analyzed by BD FACS Aria™ III Cell Sorter. Statistical analysis of corr.. View More»
DOI:
10.35248/JMCB.25.06(1).001
