The effectiveness of mild general hypothermia in reducing the sid | 49759

Journal of Neurology & Neurophysiology

ISSN - 2155-9562

The effectiveness of mild general hypothermia in reducing the side effects of delayed r-tPA treatment after embolic stroke in rats

Joint Event on 22nd International Conference on Neurology and Neurophysiology & 23rd International Conference on Neurology and Neurosurgery

April 23-24, 2018 Rome, Italy

Atoosa Alibeigi

Rafsanjan Physiopharmacology Center, Iran

Posters & Accepted Abstracts: J Neurol Neurophysiol

Abstract :

Objectives: Late alteplase (tPA)-associated complications cause narrow treatment time window of this only Food and Drug Administration (FDA) approved drug. In addition, induced hypothermia has neuroprotective properties in acute ischemic stroke, so in this study, we investigate whether general hypothermia can prevent side effect of late tPA treatment in the embolic model of stroke. Materials & Methodology: Forty male Wistar rats were randomly assigned into five groups 1: Sham, 2: Control, 3: Hypothermia, 4: r-tPA and 5: Hypothermia/r-tPA). General hypothermia (GH) was induced at 5.5 hours after induction of embolic stroke. Thirty min after initiation of cooling treatment, tPA was administrated. The infarction volume, brain edema, blood-brain barrier (BBB), matrix metallopeptidase 9 (MMP9) and neurological deficits were assessed after two days. Results: In comparison with control group, the general inducing of hypothermia decreased the infarct volume, BBB, MMP9, neurological deficit, and brain edema at 5.5 hours after stroke. In brain edema, no significant difference was observed between hypothermia and control group. In group number 5, hypothermia and r-tPA led to decrease in infarction volume, brain edema, BBB, MMP9 and neurological deficit 6 h after stroke. Conclusions: The findings of the present study suggest that general hypothermia can prevent the side effect of delayed tPA treatment in an embolic model of stroke.