Sumeet Gullaiya, Shyam Sunder Agrawal, Vishal Dubey and Varun Singh
Scientific Tracks Abstracts: J Neurol Neurophysiol
Aim:Study was undertaken to evaluate and compare the neurodegenerative defending potential of Curcumin, De- methoxycurcumin & Bis-demethoxycurcumin on 6-hydroxy dopamine induced animal Parkinsonism model. Material and Method: Isolated curcuminoids were administered (30 mg/kg) for three weeks followed by unilateral injection of 6-hydroxydopamine on 22 nd day (10μg/2μl) into the right striatum leading to extensive loss of dopaminergic cells. The behavioral observations (apomorphine induced rotations, motor coordination & locomotor activity), biochemical markers (Malondialdehyde, Glutathione, Glutathione reductase, Glutathione peroxidase, Superoxide dismutase & Catalase), Dopamine D 2 receptor binding assay and tyrosine hydroxylase immunohistochemistry were evaluated after three weeks of leison. Results & Discussion: 6- hydroxydopamine induced neurodegeneration is associated with an antioxidant deficit and deranged levels of biochemical markers. The increase in apomorphine-induced rotations and deficits in locomotor activity & muscular coordination due to 6- hydroxydopamine injection were significantly restored in curcuminoids pretreated groups with Curcumin showing maximal recuing effect as compared to protective effects of De-methoxycurcumin and Bis-demethoxycurcumin. Pretreated animals showed significant protection against neuronal degeneration compared to leison animals by normalizing the deranged levels of biomarkers and showed the effectivity in the order Curcumin > De-methoxycurcumin ≥ Bis-demethoxycurcumin. The same order of potency was observed in D 2 receptors binding assay and tyrosine hydroxylase immunohistochemistry study. Curcuminoids appears to shield progressive neuronal degeneration from increased oxidative attack in 6-OHDA lesioned rats through its free radical scavenging, MAO-B inhibiting and DA enhancing capabilities in the sequence of efficacy; Curcmin > De-methoxycurcumin > Bis-demethoxycurcumin. Further, curcuminoids may have potential utility in treatment of many more oxidative stress induced neurodegenerative disorders.