Vishal Sahu, Abhishek Gupta, Vertica Agnihotri, Anant Mohan and Sharmistha Dey
All India Institute of Medical Sciences, India
Posters & Accepted Abstracts: Oncol Cancer Case Rep
Rac proteins are found to play major role in tumorogenesis. This study aimed to quantify Rac1 and Rac1b in serum of non-small cell lung cancer (NSCLC) patients. The blood of 77 NSCLC patients and 52 healthy controls were collected and the concentration of Rac1 and Rac1b was quantified mainly by surface plasmon resonance and was verified by Western blot analysis. Statistical analyses of all the data were performed by Graph Pad Prism version 5.0. Rac1 and Rac1b both were found to be significantly over expressed in lung cancer patients compared to healthy controls. The levels of Rac proteins were found to be elevated in all stages of cancer. Despite the low survival rate, we managed to collect serum sample of the 18 follow up patients after the therapy, where 11 patients of CR+PR group showed down regulation of the Rac protein after therapy and unfortunately 80% patients died during the study period. This study first time reported the elevated level of Rac protein in serum of NSCLC patients. The high specificity and sensitivity obtained from ROC analysis for Rac1 and Rac1b envisaged it to be used as a serum diagnostic marker in early stage of cancer.
Email: vishal.sahu2007@gmail.com