Functional & Comparative Proteomics Center, USDA-ARS, Cornell University, Ithaca, NY, USA
Research
Proteomic Effects of Magnesium Stress on Biofilm Associated Proteins Isolated from Cellulolytic Bacillus licheniformis YNP5-TSU
Author(s): Joshua A O’Hair, Hui Li, Mahesh Rangu, Santosh Thapa, Yong Yang, Tara Fish, Sarabjit Bhatti, Theodore W. Thannhauser and Suping Zhou*
Optimization of cellulase activity is vital for synthesizing the end-products of second generation biofuel production. The slightest change in fermentation parameters can reduce the secretion of necessary enzymes to degrade cellulosic biomass. Determining the ecological effects of certain key media components is essential to understand how bacterial species will respond in a fluid environment. For our experiment a cellulosic media was designed to enhance the industrially important thermophile, Bacillus licheniformis YNP5-TSU. After several attempts to simplify the carboxymethylcellulose (CMC) media composition, impaired biofilm maturation and cellulase activity was noticed. This negative artifact occurred only when magnesium sulphate was removed from media. To analyze the shift in gene expression caused by magnesium stress, biofilm associated proteins were extracted from both.. View More»
DOI:
10.35248/0974-276X.19.12.504
