Department of microbiology, Government College University, Faisalabad, Pakistan
Research Article
Molecular Detection of Mycoplasma gallisepticum and Mycoplasma synoviae Infection in Poultry
Author(s): Ahmad Raza, Muhammad Waqar Mazhar*, Hira Tahir, Mamoona Sultan and Fatima Mazhar
In Pakistan, production of poultry has been evolved as a good alternate of beef and mutton. In this study multiplex PCR approaches were adopted to differentiate and detect avian mycoplasma in a single PCR reaction as a step forward in the economization of PCR detection. From the total of 25 samples, 25 (100%) had a positive Mycoplasma isolation result. The biochemical test yielded the highest number of isolations, with MG accounting for 10/25. Commercial DNA-based PCR kits have been developed as a result of recent advancements in diagnostic techniques for Mycoplasma infections. Tetracyclines, fluoroquinolones, tilmicosin, tylosin, spiramycin Infections should be reduced as much as possible... View More»
