Yuhan Gao
Bolivarian Rep. of Venezuela
Research Article
Effect of GPD2 and PDC6 Deletion on Isobutanol Titer in Saccharomyces Cerevisiae
Author(s): Aili Zhang, Yuhan Gao, Jingzhi Li and Hongxing JinAili Zhang, Yuhan Gao, Jingzhi Li and Hongxing Jin
Objectives: Isobutanol is regarded as a next-generation biofuel for its higher octane number and higher energy density than ethanol. However, during isobutanol biosynthesis, ethanol and glycerol are major unwanted byproducts. In order to improve isobutanol production in Saccharomyces cerevisiae, we used molecular biology and genetic recombination technologies to eliminate ethanol and glycerol titers.
Methods: In this study, GPD2 and PDC6 were deleted to increase isobutanol production in microaerobic fermentation of Saccharomyces cerevisiae. Engineered strain HZAL–13 (PGK1p–BAT2 gpd2Δ::RYUR) was constructed by overexpressing of BAT2 (which encodes a branched-chain amino-acid aminotransferase) and deleting GPD2 (which encodes glycerol-3-phosphate dehydrogenase). Engineered strain HZAL–14 (PGK1p–BAT2 pdc6Δ::R.. View More»