Rajiv Gandhi
Toronto Western Hospital,
East Wing 1-439, 399 Bathurst St, Toronto, Ontario, M5T 2S8
Canada
Research Article
Gene Expression Profiles of the Subcutaneous Fat and Infrapatellar Fatpad in Individuals with Early and Endstage Knee Osteoarthritis: A Cross-sectional Analysis
Author(s): Rajiv Gandhi, Amanda Weston, Carl Virtanen, Mark Takahashi, Nizar Mahomed and Anthony PerruccioRajiv Gandhi, Amanda Weston, Carl Virtanen, Mark Takahashi, Nizar Mahomed and Anthony Perruccio
Objective: Previous research has examined gene expression of the IFP from individuals with early and endstage knee OA. However, there is little understanding of whether the differential expression that was found is a local effect associated with OA disease severity. The objective of this study was to compare gene expression of the SAT and IFP both within individuals with early and late stage knee OA.
Methods: Knee SAT and corresponding IFP samples were harvested from twenty-nine patients with endstage and five patients with early stage OA at the time of knee surgery. Total RNA was then extracted, labelled and hybridized to Illumina whole genome expression arrays. Arrays were scanned and intensity of hybridization quantified. Filtered data were analyzed to find significantly different expressed genes between the disease stages and fat types... View More»
