Naomi Hachiya
Department of Neurophysiology, Tokyo Medical University, Tokyo 160-8402,
Japan
Research Article
Calmodulin-Like Skin Protein (CLSP) is a Novel Biomarker Candidate for
Pick's Disease by Unfoldin-Modified Proteomic Analysis
Author(s): Kana Nishijima, Kaoru O\'hara, Kiyotoshi Kaneko and Naomi HachiyaKana Nishijima, Kaoru O\'hara, Kiyotoshi Kaneko and Naomi Hachiya
We identified a biomarker candidate for Pick’s disease using robust protein-unfolding activity, designated Unfoldin, which was purified from yeast cells. Unfoldin localized to the cell periphery at the stationary phase, whereas it was on the budding sites during the log phase and then surrounded the bud neck during cell division. ATP, but not its hydrolysis, promoted Unfoldin binding to protein substrates and unfolded their conformation. A total of 514 Pick bodies were purified and isolated with a laser-microdissection system without a healthy brain region and solubilized with Unfoldin in the presence of ATP, and then the components were analyzed using a shotgun protein analysis by liquid chromatography-tandem mass spectroscopy. A MASCOT analysis revealed that calmodulin-like skin protein is a possible marker protein for Pick’s disease, and it was localized in the Pick bod.. View More»
