Mohamed Ouardouz
NRAC, 39 du météore Gatineau,
Quebec, J9A 3B5
Canada
Research Article
GDPbS Activates Excitatory Synapses in CA1 Pyramidal Cells by Disinhibiting the PKA Activating Pathway
Author(s): Mohamed OuardouzMohamed Ouardouz
Objective: During the early postnatal brain developmental stages, excitatory synapses mediated by AMPA
receptors are weak or silent. Activity-dependent insertion of AMPA receptors into synapses depends on the activation
of protein kinase A. In this work, we investigated the effect of Guanosine 5’-[βthiol] diphosphate (GDPßS) on excitatory
and inhibitory synaptic currents in CA1 pyramidal cells at postnatal days 9-12.
Methods: Whole-cell patch-clamp recordings from identified hippocampal CA1 pyramidal cells were used. GDPβS
Was applied through the recording electrode.
Results: GDPβS induces an increase in excitatory synaptic current amplitude, but not in the inhibitory synaptic
current amplitude. An analysis of the change in excitatory synaptic current amplitude in the presence of GDPβS revealed
a p.. View More»
