Gonzalo Montoya-Ayala
Mexico
Research Article
Human Cementoblastoma- Derived Cells Express Cartilage- Like
Phenotype In vitro and In vivo and Induce Bone Formation
Author(s): Lia Hoz-Rodriguez, Ana L Garcia-Hernandez, Enrique RomoArevalo, Fabiola Salgado-Chavarria, Gonzalo Montoya-Ayala, MargaritaZeichner-David, Rodrigo Correa-Prado, Sonia Lopez-Letayf and Higinio ArzateLia Hoz-Rodriguez, Ana L Garcia-Hernandez, Enrique RomoArevalo, Fabiola Salgado-Chavarria, Gonzalo Montoya-Ayala, MargaritaZeichner-David, Rodrigo Correa-Prado, Sonia Lopez-Letayf and Higinio Arzate
The molecular mechanisms that regulate proliferation and differentiation of cementoblasts, have not been elucidated to date. In this paper, it is shown that human cementoblastoma-derived cells (HCDC) express greater levels of cartilage markers such as type II and X collagens, aggrecan (ACAN) and SRY-box 9 (SOX9) stem cell markers; MCAM (melanoma cell adhesion molecule; synonym: CD146) and STRO-1 than human gingival fibroblasts (HGF). Our in vivo studies demonstrate that HCDC induce bone formation through endochondral ossification as observed 14 days after HCDC were implanted in rat critical-size calvarial defects. At 30 and 60 days post-implantation, the defects treated with HCDC were filled with 70 ± 1.6 and 91 ± 1.3% of newly formed bone. To confirm the identity of this tissue, we analyzed the newly formed bone using histomorphology and immunostaining. The res.. View More»
