Giridharan Nappanveettil
India
Research Article
Role of Leptin in Immune Dysfunction in WNIN Obese Rats
Author(s): Prathibha Bandaru, Hemalatha Rajkumar, Venkata Prasad Upadrasta and Giridharan NappanveettilPrathibha Bandaru, Hemalatha Rajkumar, Venkata Prasad Upadrasta and Giridharan Nappanveettil
Aim: Role of leptin in bringing about systemic immune response under obesity has so far been speculative. In the present study we addressed this question using genetically obese rats developed at our centre- WNIN/Ob and WNIN/ GR-Ob.
Methodology: Both the lean (+/+) and homozygous obese animals (-/-) received either phosphate buffered saline or leptin. While another set of fed lean and obese served as controls. Effect of leptin on immune function was assessed in terms of splenic lymphocyte proliferative response to Concavalin A (Con A), splenic CD4/CD8 ratio and Nitric oxide (NO) production by macrophages. Further, we also studied if the effect of leptin on immune function was associated with changes in receptor OBR (Leptin receptor) and or JAK2 (Janus tyrosine kinase 2) total protein expression.
Results: Lean anima.. View More»
