Alexandre Tafuri
Brazil
Research Article
Langerhans Cells Ascertaining in Cervical Tissues Obtained from Women with Cervical Intraepithelial Neoplasia
Author(s): Daniele de Souza Camargos, Alexandre Tafuri, Paula Ávila Fernandes, Marcelo Vidigal Caliari, Walderez Ornelas Dutra and Annamaria Ravara VagoDaniele de Souza Camargos, Alexandre Tafuri, Paula Ávila Fernandes, Marcelo Vidigal Caliari, Walderez Ornelas Dutra and Annamaria Ravara Vago
Objectives: Impairment of cell-mediated immunity in cervical cancer (CC) and intraepithelial neoplasia (CIN) has been reported. In this study, Langerhans cells (LC) subpopulations were quantitatively evaluated in cervical tissuesamples obtained from Brazilian patients exhibiting progressive grades of CIN, (CIN)I (n=3), CINII (n=3) and CINIII (n=3), in addition to three normal controls.
Methods: The precise number of cervical LC was determined in the entire area of lesioned epithelium from CIN samples, by performing a morphometric analysis of Langerhans cells positive for two distinct markers, S100 and Langerin (Lang) detected either by Immunofluorescence or Immunohistochemistry analysis.
Results: In normal cervix, a higher density of Langerin+ cells was observed whereas S100+ LC were predominant in pre-neop.. View More»
