Journal of Neurology & Neurophysiology

ISSN - 2155-9562


The Slow Speed Analysis Of Video-Monitoring Is Essential For The Observation Of Behavioral Manifestations In The Chronic Phase Of Pilocarpine Model In Non-Se Female Rats

Janise Dal Pai*, Rebeca Padrão Amorim, Gabriela Predebon, Ana Carolina Cossa, José Cláudio da Silva, Ivian Sanabria and Débora Amado

Only few studies have focus on animals that received Pilocarpine (Pilo) and did not develop behavioral status epilepticus (SE) and, whether they may become epileptic in the model’s chronic phase. Authors observed mossy fiber sprouting in the hippocampus of Non-SE (NSE) rats (Scharfman et al., 2001), while others observed spontaneous and recurrent seizures (SRS) 6-8 months after animals received the drug (Navarro Mora et al., 2009). Neuronal excitability is influenced by female hormones, as well as, the occurrence of SE in castrated and non-castrated female rats. However, it is not known whether females that received Pilo and did not show SE, may have SRS. The aim of this work was to investigate whether castrated and non-castrated female rats that did not show behavioral SE after Pilo, will develop SRS in the following one-year. For that, animals received 360mg/kg of Pilo and were video-monitored for 12 months. SE females from castrated and non-castrated groups became epileptic since the first month after drug injection. Epileptic behaviors were identified watching recordings in the fast speed. Castrated and Non-castrated NSE animals showed behaviors resembling seizures described by Racine Scale stages 1-3. Motor alterations of these groups were observed only when recordings were analyzed in slow speed. In addition, behavioral manifestations as, rhythmic head movements, sudden head movements, whole body movements and immobility were also observed in both, SE and NSE groups. We concluded that slow speed analysis of motor alterations was essential for the observation of NSE findings, which suggests that possibly many behaviors may be underestimated in epilepsy experimental research.