Paulo Vitor Marques Simas
The key to control emerging infectious diseases is to perform active epidemiological surveillance to identify the main healthy reservoir animals into each ecosystem. In this sense, Tadarida brasiliensis bats specie is widely distributed in the American Continent and the species and occur in the most populated areas of Brazil. This specie is adapted to urban areas, enabling contact and spread of several viral agents to humans, domestics and production animals. Some viral families like Coronavirus (CoV) stands out for sanitary and epidemiological surveillance, since highly pathogenic virus strains have evolved from bats like SARS in 2002, MERS in 2013 and probably SARS-2 current epidemic. The aim is to characterize the Coronavirus species and its phylogenetic relationships using viral metagenomics in T. brasiliensis bats’ specie, a typical specie distributed into Americas. We used anal and oral swabs of bats’ specimens collected in Jequitibás Wood, Central region of Campinas City, São Paulo State, Brazil in 2011. Samples were subjected to Next Gen-Sequencing (NGS) using the Illumina platform HiSeq 2500. Phylogenetic analyzes were performed in MEGA. The BLAST similarity search was conducted from different databases and matches were obtained with sequences of viral origin of great interest for health surveillance like unclassified Alphacoronavirus. Phylogenetic analyzes only for coronavirus matches included representative sequences of all genus of Orthocorovirinae subfamily – alpha, beta, gamma and deltaCoV and were performed using maximum likelihood methods (ML) and neighbor-joining (NJ). We identify sequences phylogenetically related AlphaCoV-like, Appalachian Ridge Cov.2, Porcine Epidemic Diarrhea Virus (PEDV), HCoV-NL63, Bat Coronavirus 1B, the viruses of importance to one health. One sample was validated using RT-PCR and Sanger Sequencing and was similar to PEDV. Considering the zoonotic impact of many CoV, our results contribute greatly to a better understanding of the molecular eco-epidemiology in the evolution of these viral agents before epidemics spillover.
