S.Thenmozhi, M.Leena Priya, Garimella Saraswathi, Sumeet Dwivedi, U.Subasini
To develop the finger print of medicinally and economically important leaves of Vitex leucoxylon Linn. Chromatographic techniques were used for separation of components from various extracts of dried leaves of Vitex leucoxylon Linn. in different solvents such as Petroleum ether, Benzene and Ethyl acetate. CAMAG HPTLC system equipped with Linomat V applicator, TLC Scanner 3, Reprostar 3 and WIN CATS -4 software were used. HPTLC finger printing of Petroleum ether extract of leaf revealed 8 peaks under 254 nm with Rf values in the range of -0.02 to 0.86 and 5 peaks under 366 nm with Rf values in the range of -0.02 to 0.86; Benzene extract of leaf revealed 10 peaks under 254 nm with Rf values in the range of -0.03 to 1.10 and 8 peaks under 366 nm with Rf values in the range of 0.03 to 0.93; Ethyl acetate extract of leaf revealed 9 peaks under 254 nm with Rf values in the range of -0.03 to 0.91 and 8 peaks under 366 nm with Rf values in the range of -0.03 to 0.79. It can be concluded that HPTLC finger printing analysis of leaf extracts of Vitex leucoxylon can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker and also a good estimator of genetic variability in plant populations.
