Dmitriy V. Kozlov, Yulia S. Korneva *, Alexander E. Dorosevich
Background: The problem of high mortality from myocardial infarction (MI) is very actual all over the world in spite of the progress in diagnostic and treatment. The great amount of investigations is directed to cell therapy, which is focused on the correction of cell populations in hurried and intact zones to stimulate reparation. In this work the investigation has been done according to the study of perivascular communicative systems.
Materials and methods: 73 hearts of dead people from MI were investigated. According to the morphology and multiplicity, they were divided into 6 groups: acute (AMI) and recurrent (RMI) MI with prescription 1-2 days (AMI-1, RMI-1); 3-5 days (AMI-2, RMI-2); more than 5 days (AMI-3, RMY-3). During the autopsy samples of cardiac muscle 1 cm 3 in capacity were cut from the centre of necrotic zone (LV-1), demarcation zone (LV-2). We accepted the center of interventricular septum (IS) as intact zone . The material was microscopically analyzed with Hematoxylin and Eosin and morphometric accounting of cells of stroma was done. For statistical treatment non parametric methods were used: U-test and Spearmen rank correlation coefficient. Probability values <0.05 were considered significant.
Results: Quantitative differences were the following: prevalence of hematogenic cells for AMI and histogenic cells for RMI. The greatest amount of differences were found in AMI-3 and RMI-3. Quantitative differences were more particular for macrophages (Mph), fibroblasts (Fb) and polymorphonuclear leucocytes (Pnl) in LV-1 - LV-2 and LV-1 - IS. Significant differences were mostly for groups AMI- 2-RMY-2. Significant differences LV-2 and IS were rare and were related to Fb, fibrocytes (Fc) and lymphocytes (Lf). Only Lf of LV-1 had a positive correlation with Lf in LV-2 and IS in all groups. In all groups mostly histiogenic cell elements had a positive correlation (Lf, Fb, Fc).
Conclusions: Differences of quantitative rates for AMI and RMI were concentrated in demarcation zone. There was a domination of the hematogenic cells elements for AMI and histogenic for RMI. The most significant differences were for AMI-2 and RMI-2. There was not a great number of correlation, probably because of a short-run process.
