Poppy Anjelisa Z. Hasibuan, Rosidah, Syafruddin Ilyas, M. Pandapotan Nasution
Objectives: to evaluate the antioxidant and cytotoxic activities of Plectranthus amboinicus, (Lour.) Spreng.
Methods: The leaves of Plectranthus amboinicus, (Lour.) Spreng. were extracted using n-hexane, aetylacetate and ethanol by maceration method. The antioxidant activity was tested using DPPH and Beta Carotene-Linoleic Acid methods, and compared to those of BHA (butylated hydroxyanisole), BHT (butylated hydroxytoluene) and quercetin. Cytotoxic assay was performed on MCF7 breast cancer cell line, using MTT [3-(4,5-dimetiltiazol-2-il)-2,5 difeniltetrazolium bromide) assay.
Results: The aetylacetate and ethanolic extracts of Plectranthus amboinicus, (Lour.) Spreng. leaves were found to exhibit antioxidant activity with IC50 value 350.74 μg/mL and 281.26 μg/mL by DPPH method. The ethanolic and aetylacetate extracts showed a high antioxidant activity by ß-Carotene-Linoleic Acid Method. N-hexane and aetylacetate extracts showed potent cytotoxic effect on MCF7 with IC50 63.644 μg/mL, 7.647 μg/mL respectively.
Conclusion: All extracts exhibited different potency in antioxidant activity which depending on extracting solvent used and secondary metabolites occur in each extract. The n-hexane and aetylacetate extracts exhibited strong cytotoxic effect that suggest to further study.
