Journal of Microbiology and Immunology


Immunomagnetic separation (IMS) is a laboratory tool that can efficiently isolate cells out of body fluid or cultured cells. It can also be used as a method of quantifying the pathogenicity of food, blood or feces. DNA analysis have supported the combined use of both this technique and Polymerase Chain Reaction (PCR).  Another laboratory separation tool is the affinity magnetic separation (AMS), which is more suitable for the isolation of prokaryotic cells.Immunomagnetic separation (IMS) is a method that deals with the isolation of cells, proteins, and nucleic acids within a cell culture or body fluid through the specific capture of biomolecules through the attachment of small-magnetized particles, beads, containing antibodies and lectins. These beads are coated to bind to targeted biomolecules, gently separated and goes through multiple cycles of washing to obtain targeted molecules bound to these super paramagnetic beads, which can differentiate based on strength of magnetic field and targeted molecules, are then eluted to collect supernatant and then are able to determine the concentration of specifically targeted biomolecules. The technique of immunomagnetic separation (IMS) obtains certain concentrations of specific molecules within targeted bacteria. A mixture of cell population will be put into a magnetic field where cells then are attached to super paramagnetic beads, specific example are Dynabeads (4.5-μm), will remain once excess substrate is removed binding to targeted antigen. Dynabeads consists of iron-containing cores, which is covered by a thin layer of a polymer shell allowing the absorption of biomolecules. The beads are coated with primary antibodies, specific-specific antibodies, lectins, enzymes, or streptavidin ; the linkage between magnetized beads coated materials is cleavable DNA linker cell separation from the beads when the culturing of cells is more desirable. Many of these beads have the same principles of separation; however, the presence and different strength s of magnetic fields requires certain sizes of beads, based on the ramifications of the separation of the cell population. The larger sized beads (>2μm) are the most commonly used range that was produced by Dynal (Dynal [UK] Ltd., Wirral, Mersyside, UK; Dynal, Inc., Lake Success, NY). Where as smaller beads (<100nm) are mostly used for MACS system that was produced by Miltenyi Biotech (Miltenyi Biotech Ltd., Bisley, Surrey, UK; Miltenyi Biotech Inc.

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