Ali Mohammad Senai
A basic, exact, precise and quick elite flimsy layer chromatographic technique has been created and approved for the synchronous estimation of two enemy of diabetic medications, sitagliptin and metformin hydrochloride in mass and tablet measurements structure. Study was performed on TLC plates precoated with silica gel 60F254 utilizing methanol: alkali: frosty acidic corrosive (9.4:0.4:0.2 v/v/v)as the versatile stage. A TLC scanner set at 214 nm was utilized for direct assessment of the chromatograms in the absorbance mode. Technique was approved by ICH rules. The relationship coefficients of alignment bends were seen as 0.999 and 0.998 in the focus scope of 100–1100 and 1000–11000 ng band1for sitagliptin and metformin, separately. The technique had an exactness of 99.70% for sitagliptin and 100.02% for metformin hydrochloride. Intra and entomb day exactness estimated as coefficient of variety were under 2% for both analytes. The restriction of identification and quantitation were 7.08 ng band1 and 21.82 ng band-1, individually for sitagliptin and 19.31 ng band-1 and 58.51 ng band-1, separately for metformin hydrochloride. The strategy could decide these medications all the while from dose structure with no impedance of the tablets excipients.
