Evaluation of the Antimicrobial Efficacy of Different Intrac | 88862

Journal of General Dentistry


Evaluation of the Antimicrobial Efficacy of Different Intracanal Medications on Enterococcus Faecalis Biofilm: A Comparative In Vitro Study

Eduardo Fernandes Marques*, Alícia Costa Santos, Anna Clara Rodrigues Barbosa, Victória Arruda Gomes, Marcelo Faria da Silva and Carina

The total elimination of microorganisms during endodontic therapy, although it is desired, is difficult to achieve. Even after canal filling, microorganisms can be in the dentin mass and periapical region. This study aims to evaluate in vitro the antibacterial efficacy of different intracanal medications on an Enterococcus faecalis biofilm. It was used reference microorganisms, Enterococcus faecalis NEWP 0012, where the bacterial suspension was seeded uniformly on Mueller Hinton (MH) agar. The plates were incubated under aerobic conditions, at a constant temperature in the range of 35°C-37°C, for 24 hours. The intracanal medications tested were: G1 Calcium hydroxide associated with 2% Chlorhexidine, G2 Calcium hydroxide associated with ozone stratum, G3 Calcium hydroxide associated with paramonochlorophenol, G4 Calcium hydroxide associated with Otosporin, G5 Calcium hydroxide, G6 2% Chlorhexidine, G7 Ozone extract, G8 Paramonochlorophenol, G9 Otosporin, G10 Saline solution, G11 Calcium hydroxide associated with iodoform, G12 Iodoform, G13 Calcium hydroxide associated with NDP, G14 NDP. After handling the materials, they were impregnated on 5 mm diameter absorbent paper discs and distributed equidistantly on the plates seeded with the microorganism. The experimental data were obtained by measuring the zone of inhibition within 24h, 72h, and 7 days. In this way, the microorganism was classified as resistant or sensitive to the different products. The test was reproduced 3 times. After analysis, it was observed that within 24 hours, 72 hours and 7 days, only the groups G1 Calcium hydroxide associated with 2% chlorhexidine (18 mm of halo of inhibition), G5 Calcium hydroxide (13 mm of halo of inhibition), G6 2% chlorhexidine (16 mm of halo of inhibition) and G9 Otosporin (9 mm of halo of inhibition) showed a halo of inhibition in the time intervals. However, the groups did not show halo of inhibition, being ineffective against E. faecalis. According to the obtained data, chlorhexidine gel 2% showed larger halos of inhibition, however, calcium hydroxide was associated with chlorhexidine gel 2%, calcium hydroxide and Otosporin. Therefore, new researches with different methods should be used to verify the efficacy of the medications used, since they presented divergence compared to other researches already carried out.